Processing and analysis of Small Angle X-ray Scattering (SAXS) data.

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BioXTAS RAW is a program for analysis of Small-Angle X-ray Scattering (SAXS) data. The software enables: creation of 1D scattering profiles from 2D detector images, standard data operations such as averaging and subtraction, analysis of radius of gyration (Rg) and molecular weight, and advanced analysis using GNOM and DAMMIF. It also allows easy processing of inline SEC-SAXS data and data deconvolution using the evolving factor analysis (EFA) method.

To install:
Download the appropriate install guide from the Files tab.

User guides:
RAW guides are available in the Files tab.

Externally hosted user guides:
• MacCHESS RAW tutorial videos:
• MacCHESS RAW tutorial data:

If you would like to use RAW on a beamline, please submit a request to have the header and/or image formats added to RAW.

Send requests, comments, and bug reports to: soren.skou.nielsen (at) gmail (dot) com and hopkins.jesse (at) gmail (dot) com.

BioXTAS RAW Web Site


  • Calibrate, mask, radially integrate, and normalize 2D images to make 1D scattering profiles
  • Average, subtract, merge, rebin, and interpolate scattering profiles
  • Easily process in-line SEC-SAXS data
  • Calculate radius of gyration (Rg) and I(0) via Guinier fit
  • Calculate the molecular weight via I(0) comparison to standards, absolute calibration, correlation volume, and corrected Porod volume
  • Run GNOM, DAMMIF, DAMAVER, DAMCLUST, and AMBIMETER (requires ATSAS installation)
  • Deconvolve SEC-SAXS data using singular value decomposition (SVD) and evolving factor analysis (EFA)
  • Calculate P(r) functions using a Bayesian indirect Fourier transform (BIFT)
  • Can read 27 image formats including: Pilatus, CBF, HDF5, Eiger, Quantum, Mar345, ESRF EDF, SAXSLab300,16 bit TIF, 32 bit TIF, Bruker, Rigaku SAXS
  • And more . . .!


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Additional Project Details

Intended Audience

Science/Research, End Users/Desktop

User Interface


Programming Language

Python, C



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