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Gemi, an automated, fast, and easy-to-use bioinformatics tool with a user-friendly interface to design primers and probes for polymerase chain reaction (PCR). Gemi accepts multiple aligned and long DNA and RNA sequences with degenerate nucleotide (non-A/C/G/T bases). Gemi can be used for quantitative, real-time and conventional PCR (qPCR, rt-PCR, etc.), and Sanger sequencing. Gemi can parse large dataset of sequences efficiently.

Python codes can be supported upon request.

Article:
Gemi: PCR primers prediction from multiple alignments
Comparative and functional genomics 2012;2012:783138
DOI: https://doi.org/10.1155/2012/783138
PMID: https://www.ncbi.nlm.nih.gov/pubmed/23316117
PMCID: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3535827/

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License

GNU General Public License version 3.0 (GPLv3)

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Additional Project Details

Operating Systems

BSD, Linux, Windows

Intended Audience

Agriculture, Education, Government, Science/Research

User Interface

.NET/Mono, Win32 (MS Windows)

Programming Language

Related Categories

C# Genetic Algorithms, C# Bio-Informatics Software, C# Education Software

Registered

2012-03-21

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