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123FASTQ performs the preprocessing of Illumina next-generation sequencing reads (FASTQ files), making the workflow easier and more accessible than ever.
Download the quick user manual for the latest version:
https://dl.adbioinformatics.net/NGSNeeds/myTools/123Fastq_v1.3_Manual.pdf
**Authors:**
Milad Eidi
Samaneh Abdolalizadeh
Mohammad Hossein Nassirpour
**Supervisors:**
Javad Zahiri, PhD
University of California San Diego
Masoud Garshasbi, PhD
Tarbiat Modares University, Tehran, Iran
If you use 123FASTQ, please cite the following preprint:
**123FASTQ: an intuitive and efficient tool for preprocessing Illumina FASTQ reads**
https://www.biorxiv.org/content/10.1101/2024.03.08.584032v1
**Important:** From now on, all future updates, releases, and related information for 123FASTQ will be made available through the project's GitHub page.
https://github.com/MiladEidi/123FASTQ
Features
- Next generation sequencing reads quality check
- Multithreaded QC implemented
- Quality check of paired-end reads
- Flexible trimmer
- Trim recommendations based on quality check results
- Adapter decontamination by two approaches
- Fast5 to FASTQ conversion
- SAM/BAM to FASTQ conversion
- Cross platform (unix, windows)
- Written in java
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