Search Results for "genome annotation"
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Showing 73 open source projects for "genome annotation"
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kSNP
kSNP4 does SNP discovery and SNP annotation from whole genomes
kSNP4 identifies the pan-genome SNPs in a set of genome sequences, and estimates phylogenetic trees based upon those SNPs. SNP discovery is based on k-mer analysis, and requires no multiple sequence alignment or the selection of a reference genome, so kSNP4 can take 100's of microbial genomes as input. A SNP locus is defined by an oligo of length k surrounding a central SNP allele. kSNP4 can analyze both complete (finished) genomes and unfinished genomes in assembled contigs or raw... -
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NGSEP
NGSEP (Next Generation Sequencing Experience Platform)
NGSEP is an integrated framework for analysis of short and long DNA high throughput sequencing reads. The current version provides functionalities for both de-novo and reference guided analysis of sequencing data, including genome assembly, read mapping, variants detection and genotyping and de-novo analysis of data generated from reduced representation protocols. NGSEP also provides modules for analysis of genomic variation databases (VCF files), including functional annotation, filtering... -
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Manatee is a web-based gene evaluation and genome annotation tool. Manatee can store and view annotation for prokaryotic and eukaryotic genomes.
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CpDB
Relational database schema and tools for bacterial genome annotation
This software allows us to create a relational database in PostgreSQL hosting full bacterial genomes. Besides the database, there is software, like a parser, to convert EMBL or GBK files to the CpDB relational schema. Once in the CpDB, one can extract unlimited reports from bacterial genomes using SQL. This software is part of the Ph.D. in Bioinformatics from Anderson Santos (https://orcid.org/0000-0003-3418-0823) developed under the Corynebacterium pseudotuberculosis (Cp) pangenome project.... -
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A fungal genome annotation pipeline (funnote) integrated by CAIQ (Chinese Academy of Inspection and Quarantine). ## prerequisites 1. skewer (https://github.com/relipmoc/skewer) 2. BLAST (https://blast.ncbi.nlm.nih.gov/) 3. DIAMOND (https://github.com/bbuchfink/diamond) 4. funannotate (https://github.com/nextgenusfs/funannotate) 5. InterProScan (https://github.com/ebi-pf-team/interproscan) 6. emapper (https://github.com/eggnogdb/eggnog-mapper)
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sRNAWorkbench
The UEA sRNA Workbench
A suite of tools for analysing small RNA (sRNA) data from Next Generation Sequencing devices. Including expression profiling of known mirco RNA (miRNA), identification of novel miRNA in deep-sequencing data and identification of other interesting landmarks within high-throughput genetic data -
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PAN2HGENE, a computational tool that allows identification of gene products missing from the original genome sequence, with automated comparative analysis for both complete and draft genomes, can be used to address this limitation. In this study, PAN2HGENE was used to identify new products, resulting in altering the alpha value behavior in the pangenome without altering the original genomic sequence. Our findings indicate that this tool represents an efficient alternative for comparative...
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misc genomics tools
Various scripts used in sequencing, annotation and RNAseq analysis
This is a collection of various programs developed in the course of a genomics project, involving the Pseudomonas strain NCIMB10586 These include * identifying and correcting errors in an (eg) pacbio genome sequence using illumina reads * prokaryotic sequence/genome annotation * RNAseq analysis - normalisation and collation of multiple samples as a group * RNAseq visualisation All scripts are provided on a 'best efforts' basis, however due to various system changes I do not guarantee... -
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miRDP2
Accurately and fast analyzing microRNAs transcriptome in plants
miRDeep-P2 (miRDP2) is developed to accurately and fast analyze microRNAs (miRNAs) transcriptome in plants. It is adopted from miRDeep-P (miRDP) with new strategies and overhauled algorithm. We have tested miRDP2 to analyze miRNA transcriptomes in such plants with gradually increased genome size as Arabidopsis, rice, tomato, maize and wheat. Compared with miRDeep-P and several other computational tools, miRDP2 processed NGS data with superior speed. By incorporating newly updated plant miRNA... -
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RNA@
RNA annotation tool
RNA@ is a RNA annotation tool for prokaryotic organisms based on a lightened Aho-Corasick like algorithm. The algorithm principle is to encode a data set of nucleotide sequences into a Finite State Machine able to recognize every occurrence of every nucleotide sequence into a genome in a single read of the genome. As nucleotide sequence data set, RNA@ uses a collection of about 300,000 RNA deposited into the NCBI. These RNA are automatically collected from about 25,000 complete deposited... -
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CODON SOFTWARE
The dataset and codon project files are available here.
Genome annotation conceptually consists of inferring and assigning biological information to gene products. Over the years, numerous pipelines and computational tools have been developed aiming to automate this task and assist researchers in gaining knowledge about target genes of study. However, even with these technological advances, manual annotation or manual curation is necessary. To assist with this problem, we present CODON, a tool for the prediction, annotation, and manual curation... -
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CusVarDB
CusVarDB generated variant protein database from NGS-datasets
CusVarDB is a windows based tool for creating a variant protein database from Next-generation sequencing datasets. The program supports variant calling for Genome, RNA-Seq and ExomeSeq datasets. The program performs mainly 4 modules 1. Align the datasets with reference database 2. Perform the variant calling using Genome Analysis Toolkit (GATK) 3. Annotate the variant using ANNOVAR 4. Create the variant protein database Apart from the main modules, the program also supports... -
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ImproveAssembly
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0206
.... This is also observed for complete genomes. Thus, we developed ImproveAssembly, a tool capable of identifying new products missing from genomic sequences, which can be used for complete and draft genomes. The identified products can improve the annotation of complete genomes and drafts while significantly reducing the bias when the information is used in other omics analyses. -
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OncoBase
A platform for decoding regulatory somatic mutations in human cancers
Whole-exome and whole-genome sequencing have revealed millions of somatic mutations associated with different human cancers. The vast majority of identified somatic mutations are located outside of coding sequences, making it challenging to directly interpret their functional effects. With the rapid advances in high-throughput chromosome conformation capture (3C)-based technologies, genome-scale long-range chromatin interactions were detected, and distal target genes of regulatory elements were... -
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The ChIP-Seq software provides methods for the analysis of ChIP-seq data and other types of mass genome annotation data. The most common analysis tasks include positional correlation analysis, peak detection, and genome partitioning into signal-rich and signal-depleted regions.
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MitoZ: A toolkit for mitochondrial genome assembly, annotation and visualization. see https://github.com/linzhi2013/MitoZ
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PWMScan
A Web-based genome-wide Position Weight Matrix (PWM) Scanner
... a PWM, the background probabilities for the letters of the DNA alphabet, and a threshold score or a p-value. The search is carried out across the entire genome sequence. It can accept PWMs, such as those available in the Transfac or Jaspar databases as well as plain-text PWMs. It computes all occurrences of the PWM in the genome sequence for a given p-value threshold or cut-off. The match list is provided in various genome annotation formats, including BEDdetail, FPS, and SGA. -
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Neopepsee
Accurate genome-level prediction of neoantigens by harnessing sequence
we developed Neopepsee, a method that applies a machine learning to classify personal immunogenicity with next generation sequencing data. -
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FEATnotator is a light weight, fast and easy to use, open source software program that annotates different kinds of next generation analysis data based on known reference genome annotation information. For SNP data, it also predicts the effect of SNPs in the protein coding regions on the codons involved and marks SNPs potentially affecting start and stop codons and splice sites. It also identifies features of user’s interest, such as genes, or biomarkers in the vicinity of the features...
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GAPE
proteogenomic analysis software
GAPE is a one-stop proteogenomic informatics software that provides a multifaceted and standard workflow against eukaryotes in proteogenomic data-analysis cycle for genome refinement and global identification of PTM events. This software allows concurrent querying of proteomic and genomic databases to refining the genome and proteome annotations comprehensively. This includes MS data and database construction, database searches, FDR calculations, statistical result integration, validation... -
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MethyMer
Design of specific primer combinations for bisulfite sequencing
... (microarrays) and gene expression (RNA-Seq) data, as well as methylation-expression correlation analysis results for 20 human cancer types. ENCODE genome regions annotation data are also integrated in MethyMer -
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Genix: Bacterial Genome Annotation Pipel
A automated annotation pipeline for bacteria / archea genomes
Genix is an online automated pipeline for bacterial genome annotation that integrates the programs Prodigal, BLAST, RNAmmer, tRNAscan-SE, INFERNAL, ARAGORN and HMMER, and the databases Uniprot, Antifam and Rfam. -
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wgssat_nbfgr
WGSSAT: SSR Annotation Pipeline
WGSSAT provides a Graphical User Interface pipeline to mine and characterize SSR from Whole genome data. This pipeline integrates prediction of genes, ncRNA, repeats and SSR from whole genome assembly and mapping of these predicted SSR on to the genome (classified according to genes, ncRNA, repeats, exonic and intronic region) along with primer designing and mining of cross-species amplification markers. The mining of SSRs from whole genome provides valuable information on the abundance of SSRs... -
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Ub_ISAP
A streamlined UNIX pipeline for mining unique viral vector integration
... integration site identification and annotation of both single and paired-end sequencing reads. Reads that contain viral sequences of interest are selected and aligned to the host genome, and unique integration sites are then classified as transcription start site-proximal, intragenic or intergenic. Ub-ISAP provides a reliable and efficient pipeline to generate large datasets for assessing the safety and efficiency of integrating vectors in clinical settings, with broader applic -
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ConsPred
ConsPred is a pipeline combining several gene/CDS annotation tools
ConsPred is a prokaryotic genome annotation framework that performs various intrinsic gene predictions, homology searches, predictions of non-coding genes, and complex features and integrates all evidence into a consensus annotation. ConsPred achieves high-quality and comprehensive annotations based on rules and priorities, similar to decision making in manual curation. Parameters controlling the annotation process are configurable by the user and the framework can be easily extended...