VividSTORM

VividSTORM is a free and open-source standalone software with graphical user interface, for the correlated visualization and analysis of superresolution single molecule localization microscopy (SMLM) molecule lists and conventional pixel intensity-based images.
The localization points (LPs) within this ROI can be analyzed using the selected built-in functions.

NOTE: If you encounter issues not addressed by the user guide, please contact by message on this site or via e-mail for additional support: vividstorm[at]koki.mta.hu

If you find our software useful in your project, please cite:
Correlated confocal and super-resolution imaging by VividSTORM
Nature Protocols 11, 163–183 (2016)
doi:10.1038/nprot.2016.002

Features

Filtering of SMLM coordinates
Manual and automatic (MACWE) ROI selection
Measurement of molecular abundance
DBSCAN clustering on SMLM data
Internalization index calculation
Intermolecualr (Euclidean and surface) distances
Surface density measurements

Project Samples

Graphical user interface of VividSTORM a) Starting the program opens three windows. Molecule lists and pixel-based images are loaded into the “STORM images” window and the “Confocal images” window, respectively. The “Plot” window displays the overlay of the active channels from both selected images. The user can pan and zoom the plot using the mouse, toggle the display of channels, and select colors for each channels.

Project Activity

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License

GNU General Public License version 3.0 (GPLv3)

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Additional Project Details

Operating Systems

Linux, Windows

Intended Audience

Science/Research, Advanced End Users

User Interface

Programming Language

Python

Related Categories

Python Data Visualization Software

Registered

2015-10-10

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