1. What is NgAgo-gDNA bioinformatic tool?
Natronobacterium gregoryi Argonaute (NgAgo) is a DNA-guided endonuclease suitable for genome editing in human cells. NgAgo binds 5' phosphorylated single-stranded guide DNA (gDNA) of ∼24 nucleotides, efficiently creates site-specific DNA double-strand breaks when loaded with the gDNA. The NgAgo-gDNA system does not require a protospacer-adjacent motif (PAM), as does Cas9(Gao et al., 2016 Nat Biotechnol). Based on these features, NgAgo-gDNA was developed, which is a bioinformatic tool for fast designing gDNA and evaluating potential off-target cleavage sites.

2. Where is the homepage of NgAgo-gDNA?
www.ngago-gdna.org (in prepare)
or http://www.biootools.com/col.jsp?id=178

NgAgo-gDNA design source code free for academic, nonprofit, and personal use.

If you use this program in your research, please cite:
Xie et al., 2016. NgAgo-gDNA: a tool for fast designing gDNA and evaluating potential off-target cleavage sites. In prepare.

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2016-05-20
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