mfsizes

A simple command-line utility to calculate biological sequence (DNA or protein) sizes in a (multi) FASTA file. It gives averages, GC (or methionine) content, N50, N90, N95, number of N's, and total bases, and can also report by codon if requested.

Features

sequence sizes (DNA or protein)
GC content, in percentage (for each sequence and overall weighted average)
methionine content, in absolute number and percentage (protein only)
codon GC content (DNA only)
multi FASTA input files
reports average sequence size, total nucleotides, N50, N90, and N95
by default, report shows sequence names sorted in descending size order
report is tab-delimited text with results from one FASTA entry per line
gzip-compressed input supported

Project Activity

See All Activity >

License

GNU General Public License version 3.0 (GPLv3)

Follow mfsizes

mfsizes Web Site

nel_h2

AI-powered service management for IT and enterprise teams

Enterprise-grade ITSM, for every business

Give your IT, operations, and business teams the ability to deliver exceptional services—without the complexity. Maximize operational efficiency with refreshingly simple, AI-powered Freshservice.

Try it Free

Rate This Project

User Reviews

Be the first to post a review of mfsizes!

Additional Project Details

Intended Audience

Science/Research

User Interface

Command-line

Programming Language

Perl

Related Categories

Perl Bio-Informatics Software

Registered

2011-05-20

Similar Business Software

Evo 2

Evo 2 is a genomic foundation model capable of generalist prediction and design tasks across DNA, RNA, and proteins. It utilizes a frontier deep learning architecture to model biological sequences at single-nucleotide resolution, achieving near-linear scaling of compute and memory relative to...

See Software
Evo Designer

Evo Designer is an advanced tool developed by the Arc Institute, leveraging the capabilities of the Evo 2 genomic foundation model to facilitate DNA sequence generation and analysis. This platform enables users to input nucleotide sequences or specify organisms, prompting the model to generate...

See Software
Genome Analysis Toolkit (GATK)

Developed in the Data Sciences Platform at the Broad Institute, the toolkit offers a wide variety of tools with a primary focus on variant discovery and genotyping. Its powerful processing engine and high-performance computing features make it capable of taking on projects of any size. The GATK...

See Software
MEGA

MEGA (Molecular Evolutionary Genetics Analysis) is a powerful and user-friendly software suite designed for analyzing DNA and protein sequence data from species and populations. It facilitates both automatic and manual sequence alignment, phylogenetic tree inference, and evolutionary hypothesis...

See Software
Correlation Engine

Correlation Engine is an interactive omics knowledgebase that puts private omics data in a biological context with highly curated public data. One of the largest biological databases in the world, Correlation Engine provides life science researchers with unprecedented access to vast numbers of...

See Software
BioTuring Browser

Explore hundreds of curated single-cell transcriptome datasets, along with your own data, through interactive visualizations and analytics. The software also supports multimodal omics, CITE-seq, TCR-seq, and spatial transcriptomic. Interactively explore the world's largest single-cell expression...

See Software