MethylExtract is a user friendly tool to generate i) high quality, whole genome methylation maps and ii) to detect sequence variation within the same sample preparation. The program is implemented into a single script and takes into account all major error sources: sequencing errors, bisulfite failure, clonal reads and single nucleotide variants. MethylExtract detects variation (SNVs – Single Nucleotide Variation) in a similar way than VarScan, a very sensitive method extensively used in SNP and genotype calling based on non-bisulfite treated reads.

Features

  • Methylation and SNV calling from NGS BS-Seq experiments
  • Detects and removes potential PCR duplicates
  • Putative bisulfite failures detection
  • Sequencing errors control
  • Statistical bisulfite failure assessment
  • Bisulfite SAM / BAM files processing
  • Multithreading and memory control

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Categories

Bio-Informatics

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Additional Project Details

Operating Systems

Linux

Programming Language

Perl

Related Categories

Perl Bio-Informatics Software

Registered

2013-08-25