MetEx

Liquid chromatography–high resolution mass spectrometry (LC-HRMS) is the most popular platform for untargeted metabolomics methods, but annotating LC-HRMS data is a long-standing bottleneck that we are facing since years ago in metabolomics research. A wide variety of methods have been established to deal with the annotation issue. To date, however, there is a scarcity of efficient, systematic, and easy-to-handle tools that are tailored for metabolomics and exposome community. So we developed a user-friendly and powerful software/webserver, MetEx, to both enable implementation of classical peak detection-based annotation and a new annotation method based on targeted extraction algorithms. The new annotation method based on targeted extraction algorithms can annotate more than 2 times metabolites than classical peak detection-based annotation method because it reduces the loss of metabolite signal in the data preprocessing process.