Nowadays, the manual cell counting, performed directly by looking through the microscope’s oculars by using a tally counter and a hemocytometer, still is the gold standard method to count Live&Dead cells (Trypan Blue is typically used to stain the dead cells). However, performing the cell counting by tagging the cells in a steady image mosaic increases the reliability of the counts performed. Unfortunately, to build a mosaic of a hemocytometer's grid shows some problem due to the repeated patterns (i.e., the squared blocks of the grid). GridMos is an open-source software tool conceived to build an image mosaic of a hemocytometer's grid. It is optimized to build the mosaic starting from a sequence of overlapping images previously acquired. The Graphical User Interface (GUI) implemented makes GridMos a very user-friendly software tool.
Requirements: MATLAB 2009b (32-bit) and Image Processing Toolbox 8.1 or later versions.
- User friendly MATLAB GUI
- Accurate blending stitching
- Specific for hemocytometer images
- To improve cell counting