CRISPR-Cas9 is a unique technology that enables geneticists and medical researchers to edit parts of the genome by removing, adding or altering sections of the DNA sequence. It is faster, cheaper and more accurate than previous techniques of editing DNA and has a wide range of potential applications. Here, a cost-effective and high-throughput strategy was developed for identifying CRISPR/Cas-induced mutations via CRISPR Amplicon Sequencing (Xie’s lab, http://www.biootools.com/). In particular, we have developed a new bioinformatics tool named CRISPRamplicon, which can be used for analysis of CRISPR/Cas9 genome editing outcomes for high-throughput sequencing data with fast speed.
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