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AutoPrimer3-3.0.2-1-x86_64.pkg.tar.xz (59.3 MB)
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| Name | Modified | Size | Downloads / Week |
|---|---|---|---|
| Parent folder | |||
| code | 2012-05-01 | ||
| ap3_win_readme.txt | 2012-05-01 | 2.0 kB | |
| instructions.pdf | 2012-05-01 | 216.2 kB | |
| auto primer3.exe | 2012-05-01 | 983.4 MB | |
| Totals: 4 Items | 983.7 MB | 0 | |
INSTALLATION: Simply copy the Auto Primer3.exe file to a directory of your choosing. The full size of the executable is 984.4 MB. INFO: Instructions are available via the program's help menu. This application invokes primer3 (http://primer3.sourceforge.net/) in order to design primers to amplify exons of human RefSeq genes. The hg19 build of the human genome is used as the genomic reference sequence. dbSNP135 reference files were used to allow a program specific mask of SNPs from primer designs (build 131 or with a MAF of 1 % or higher). The default parameters are set to enable standard PCR amplification and Sanger sequencing of exons, with product sizes of around 500 bp or smaller with at least 50 bp between the primer and the exon target. Auto Primer3 does use the human repeats mispriming library to avoid designing to repetitive regions. However, it does not check for pseudogenes or blat the primers to ensure they do not match multiple locations. As yet, only human genes are supported. Once the reference gene table is 30 days old the program will prompt you to ask if it may download the current version from the UCSC genome browser. Upon starting this program, users will notice the presence of a command prompt window as well as the main interface. Because this program invokes the primer3_core.exe command line program to perform the primer design the alternative would be much more intrusive and distracting command prompt windows popping-up for each amplicon design. As such, it was decided that a single ever present command prompt was the lesser of the two evils where it could at least display some useful information as the program progresses. CREDIT AND PERMISSIONS: primer3 is open source software available at http://primer3.sourceforge.net/ The rest of the application was written by Dr David Parry at the University of Leeds and is distributed free of charge under the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License.