Design PCR primers from DNA sequence. Widely used (190k Google hits for "primer3"). From mispriming libraries to sequence quality data to the generation of internal oligos, primer3 does it. C&perl. Developers/testers/documenters needed.
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Bio-InformaticsLicense
GNU General Public License version 2.0 (GPLv2)Follow Primer3 - PCR primer design tool
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User Reviews
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5 out of 5 starsPrimer3 works perfectly.
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5 out of 5 starsPrimer3 is perfect! Thanks.
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5 out of 5 starsI use this every single day! I really liked it.
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5 out of 5 starsmust have app for people on the go.
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5 out of 5 starsIt is an awesome small and easy to use program