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Re: [proteowizard-support] Issue with Waters MSe conversion
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From: Chambers, M. <mat...@gm...> - 2023-07-11 16:19:14
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When you see a spiky TIC like that it's indeed usually because there's two or more different functions/scan events interleaved. For MSe that's certainly the low energy and high energy functions (the latter being considered as "MS2" but only out of convention since it doesn't have a real precursor). On 7/11/2023 12:08 PM, Brian Pratt wrote: > I’d recommend eyeballing the mzXML - you’ll find that it’s all MS1, I think. How mzmine deals with displaying that, I don’t know. > >> On Jul 11, 2023, at 8:05 AM, Taylor, MJC, Mnr [mt...@su...] <mt...@su...> wrote: >> >> >> >> Thanks. So the chromatogram is then a blend of MS1 data at the top of the peaks and MS2 data at the bottom of the peaks? >> >> >> *From:*Brian Pratt bs...@pr... >> *Sent:* Tuesday, July 11, 2023 5:02 PM >> *To:* Taylor, MJC, Mnr [mt...@su...] <mt...@su...> >> *Cc:* su...@pr... >> *Subject:* Re: [proteowizard-support] Issue with Waters MSe conversion >> >> CAUTION: This email originated from outside the Stellenbosch University network. Do not click links or open attachments unless you >> recognize the sender and know the content is safe. >> >> That’s correct. Note that the window is extremely wide - it’s an all ions fragmentation. There’s no single precursor per se, but mzXML >> expects some value there. >> >> Sent from my phone, with apologies for brevity and strange autocorrections >> >> >> >> On Jul 11, 2023, at 6:05 AM, Taylor, MJC, Mnr [mtaylor--- via proteowizard-support <pro...@li...> wrote: >> >> >> >> Good afternoon, >> >> I am having some issues converting my Waters MSe data to mzXML. As can be seen in the screenshot below, the Precursor ion for all >> scans is 770. The raw data from a Waters G2 qTOF was converted using MSConvert 3.0.23191-2fd18c2 using 64-bit precision with Peak >> Picking as the only subset. >> >> Please can you comment. >> >> Thanks >> >> Malcolm Taylor >> >> University of Stellenbosch >> >> <image001.png> >> >> The integrity and confidentiality of this email are governed by these terms. Disclaimer >> <https://www.sun.ac.za/emaildisclaimer/default.aspx> >> Die integriteit en vertroulikheid van hierdie e-pos word deur die volgende bepalings bereël. Vrywaringsklousule >> <https://www.sun.ac.za/emaildisclaimer/default.aspx> _______________________________________________ >> proteowizard-support mailing list >> pro...@li... >> https://lists.sourceforge.net/lists/listinfo/proteowizard-support >> > > > _______________________________________________ > proteowizard-support mailing list > pro...@li... > https://lists.sourceforge.net/lists/listinfo/proteowizard-support |
