Re: [proteowizard-support] SCIEX *.wiff vs *.wiff2

[Chambers, M. <mat...@gm...>]

Hi Tyler,

WIFF2 and WIFF should theoretically produce the same m/z and intensity values. I don't actually have any non-SRM data to test that theory on 
though. Can you provide some that is exhibiting the difference and also what settings you're using?

Thanks,
-Matt


On 5/23/2023 6:09 PM, tfl...@gn... wrote:
>
> Hi there,
>
> I contacted you several months ago about converting waters data (thanks for your help), but now I am wondering if you might be able to 
> help me with some issues processing sciex data. It seems if I convert ZenoTof data using the *.wiff files I get different spectral data 
> that the *.wiff2 files. I haven’t looked at the exact differences, but it appears the m/z values are nearly the same (32bit float 
> imprecision ) but the intensity values from the *.wiff2 are about half of that from the *.wiff. Do you have any recommendations on 
> settings to use when converting Sciex data from either the TripleTof6000 or the Zenotof7600? We are also currently in contact with Sciex 
> to see what their recommendations are.
>
> Thank you,
>
> Tyler
>
>