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From: Fostel, J. (NIH/N. [C] <fo...@ni...> - 2008-06-05 14:13:10
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Hepatectomy Objective: remove portion of liver and observe gene expression markers in blood as it regrows move cage of rats into necropsy room remove one rat and anesthesize with nembutol. while the rat is unconscious, move to heated pad in operating cabinet shave a patch 2 inch square on the lower right of abdomen sterilize with surface treatment of ethanol carefully make 1 inchlong incision over the liver surgically dissect the left and right lobes of the liver and remove close the incision with Michel wound clips allow the rat to return to consciousness in single cage observe the rat as it regains consciousness if the rat is behaving normally, return to cagemates if not, continue to observe. if rat is in distress after several hours euthanize with nembutol and an excess of CO2 and then cervical dislocation. Sham Hepatectomy Objective: provide a comparator for rats undergoing hepatectomy move cage of rats into necropsy room remove one rat and anesthesize with nembutol. while the rat is unconscious, move to heated pad in operating cabinet shave a patch 2 inch square on the lower right of abdomen sterilize with surface treatment of ethanol carefully make 1 inchlong incision over the liver surgically move the left and right lobes of the liver close the incision with Michel wound clips allow the rat to return to consciousness in single cage observe the rat as it regains consciousness if the rat is behaving normally, return to cagemates if not, continue to observe. if rat is in distress after several hours euthanize with nembutol and an excess of CO2 and then cervical dislocation. Abdominal surgical incision Objective: create abdominal wound and observe healing process in local tissue move cage of rats into necropsy room remove one rat and anesthesize with nembutol. while the rat is unconscious, move to heated pad in operating cabinet shave a patch 2 inch square on the lower right of abdomen sterilize with surface treatment of ethanol carefully make 1 inchlong incision over the liver close the incision with Michel wound clips allow the rat to return to consciousness in single cage observe the rat as it regains consciousness if the rat is behaving normally, return to cagemates if not, continue to observe. if rat is in distress after several hours euthanize with nembutol and an excess of CO2 and then cervical dislocation. Sham surgical incision Objective: create comparator for abdominal wound healing observation move cage of rats into necropsy room remove one rat and anesthesize with nembutol. while the rat is unconscious, move to heated pad in operating cabinet shave a patch 2 inch square on the lower right of abdomen sterilize with surface treatment of ethanol allow the rat to return to consciousness in single cage observe the rat as it regains consciousness if the rat is behaving normally, return to cagemates if not, continue to observe. if rat is in distress after several hours euthanize with nembutol and an excess of CO2 and then cervical dislocation. Hair re-growth Objective: remove abdominal hair and observe re-growth process Objective: create abdominal wound and observe healing process in local tissue move cage of rats into necropsy room remove one rat and anesthesize with nembutol. while the rat is unconscious, move to heated pad in operating cabinet shave a patch 2 inch square on the lower right of abdomen sterilize with surface treatment of ethanol allow the rat to return to consciousness in single cage observe the rat as it regains consciousness if the rat is behaving normally, return to cagemates if not, continue to observe. if rat is in distress after several hours euthanize with nembutol and an excess of CO2 and then cervical dislocation. |