[Obi-plan-branch] use case

[Fostel, J. (NIH/N. [C] <fo...@ni...>]

Hepatectomy
Objective:  remove portion of liver and observe gene expression markers in blood as it regrows
move cage of rats into necropsy room
remove one rat and anesthesize with nembutol.
while the rat is unconscious, move to heated pad in operating cabinet
shave a patch 2 inch square on the lower right of abdomen
sterilize with surface treatment of ethanol
carefully make 1 inchlong incision over the liver
surgically dissect the left and right lobes of the liver and remove
close the incision with Michel wound clips
allow the rat to return to consciousness in single cage
observe the rat as it regains consciousness
if the rat is behaving normally, return to cagemates
if not, continue to observe.
if rat is in distress after several hours euthanize with nembutol and an excess of CO2 and then cervical dislocation.


Sham Hepatectomy
Objective:  provide a comparator for rats undergoing hepatectomy
move cage of rats into necropsy room
remove one rat and anesthesize with nembutol.
while the rat is unconscious, move to heated pad in operating cabinet
shave a patch 2 inch square on the lower right of abdomen
sterilize with surface treatment of ethanol
carefully make 1 inchlong incision over the liver
surgically move the left and right lobes of the liver
close the incision with Michel wound clips
allow the rat to return to consciousness in single cage
observe the rat as it regains consciousness
if the rat is behaving normally, return to cagemates
if not, continue to observe.
if rat is in distress after several hours euthanize with nembutol and an excess of CO2 and then cervical dislocation.


Abdominal surgical incision
Objective:  create abdominal wound and observe healing process in local tissue
move cage of rats into necropsy room
remove one rat and anesthesize with nembutol.
while the rat is unconscious, move to heated pad in operating cabinet
shave a patch 2 inch square on the lower right of abdomen
sterilize with surface treatment of ethanol
carefully make 1 inchlong incision over the liver
close the incision with Michel wound clips
allow the rat to return to consciousness in single cage
observe the rat as it regains consciousness
if the rat is behaving normally, return to cagemates
if not, continue to observe.
if rat is in distress after several hours euthanize with nembutol and an excess of CO2 and then cervical dislocation.

Sham surgical incision
Objective:  create comparator for abdominal wound healing observation
move cage of rats into necropsy room
remove one rat and anesthesize with nembutol.
while the rat is unconscious, move to heated pad in operating cabinet
shave a patch 2 inch square on the lower right of abdomen
sterilize with surface treatment of ethanol
allow the rat to return to consciousness in single cage
observe the rat as it regains consciousness
if the rat is behaving normally, return to cagemates
if not, continue to observe.
if rat is in distress after several hours euthanize with nembutol and an excess of CO2 and then cervical dislocation.


Hair re-growth
Objective:  remove abdominal hair and observe re-growth process
Objective:  create abdominal wound and observe healing process in local tissue
move cage of rats into necropsy room
remove one rat and anesthesize with nembutol.
while the rat is unconscious, move to heated pad in operating cabinet
shave a patch 2 inch square on the lower right of abdomen
sterilize with surface treatment of ethanol
allow the rat to return to consciousness in single cage
observe the rat as it regains consciousness
if the rat is behaving normally, return to cagemates
if not, continue to observe.
if rat is in distress after several hours euthanize with nembutol and an excess of CO2 and then cervical dislocation.