Re: [micro-manager-general] Andor iXon+, iXon3 bug?, need help

[Pedro A. <pa...@gm...>]

Hi Lukas,

Just as a sanity check, have you set the circular memory buffer to an
appropriate size?
https://micro-manager.org/wiki/Micro-Manager_Configuration_Guide#Memory_Settings


Kind regards,
Pedro Almada

On Wed, 11 Mar 2020 at 18:04, Lukas Hille <Luk...@im...> wrote:

> Hi,
>
> I need help with two Andor cameras.
>
> Two Andor cameras are used in dual view (Multi Camera adapter with
> Andor: iXon+ and Andor: iXon3).
> Acquisition is done by a burstAcquisition macro (equal to the script),
> but the described behavior applies also to MDA.
> With Frame transfer enabled and at higher Acquisition speeds (binning 2,
> 0.9us vertical clock, 20ms exposure, full ROI) i get an buffer overflow.
>
> It appears that the device adapter before 14.April2019 "old" returns
> very quick from mmc.popNextTaggedImage() and therefore produces no
> buffer overflow, while the adapter after 14.April2019 "new" takes a very
> long time to return from this function.
> This also occurs with only one Camera in use, but with two cameras the
> return time is to long to handle the data flow.
>
> A second issue is, that with Multi Camera adapter, the camera referring
> to channel 0 (independent which one) stops acquiring after some time
> (~60.000 images in Trigger: software).
> This applies to the "old" and the "new" Andor device adapter.
>
> With Multi Camera adapter and external trigger (one source for both
> cameras, Trigger set to External on both cameras), the camera referring
> to channel 0 stops acquiring within 0 to some thousand images)
>
> I don't get any debugging error message with the new adapter.
> With the old adapter i get for example: "GetNumberNewImages error:
> first: 3473 last: 3473" for the camera on channel 0 before it stops
> working.
>
> The goal would be to acquire about 200,000 time points with external
> trigger at 20ms exposure time.
>
> I tried to understand the differences in the code from commits on
> 14.April, but I didn't figure out why this makes a difference for the
> popNextTaggedImage() function.
> Also: the error message "GetNumberNewImages error:" doesn't make any
> sense to me, referring to the situation that no new images are acquired.
>
> I would be very happy to get assistance on this one.
>
> Software:
> AndorDriverPack2 - 2.103.30031.0
> Micro-Manager 2.0.0-gamma1 20200222
> mmgr_dal_Andor.dll from 1.04.2019, 16.04.2019 and 22.02.2019 (tried many
> many more)
>
> best
> Lukas
>
> --
> ____________________________________________
> Lukas Hille, M.Sc.
> microscopy engineer
> Luk...@im...
> M +43 660 349 169 3
>
> Department of Neurobiology, University of Vienna
> Campus-Vienna-Biocenter 1
> 1030 Vienna
> AUSTRIA
>
> https://neuro.univie.ac.at/
> https://www.imp.ac.at/groups/manuel-zimmer/
> Part of Vienna BioCenter
> www.viennabiocenter.org
> ____________________________________________
>
>
>
> _______________________________________________
> micro-manager-general mailing list
> mic...@li...
> https://lists.sourceforge.net/lists/listinfo/micro-manager-general
>