[micro-manager-general] Andor iXon+, iXon3 bug?, need help
Status: Beta
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From: Lukas H. <Luk...@im...> - 2020-03-11 18:02:18
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Hi, I need help with two Andor cameras. Two Andor cameras are used in dual view (Multi Camera adapter with Andor: iXon+ and Andor: iXon3). Acquisition is done by a burstAcquisition macro (equal to the script), but the described behavior applies also to MDA. With Frame transfer enabled and at higher Acquisition speeds (binning 2, 0.9us vertical clock, 20ms exposure, full ROI) i get an buffer overflow. It appears that the device adapter before 14.April2019 "old" returns very quick from mmc.popNextTaggedImage() and therefore produces no buffer overflow, while the adapter after 14.April2019 "new" takes a very long time to return from this function. This also occurs with only one Camera in use, but with two cameras the return time is to long to handle the data flow. A second issue is, that with Multi Camera adapter, the camera referring to channel 0 (independent which one) stops acquiring after some time (~60.000 images in Trigger: software). This applies to the "old" and the "new" Andor device adapter. With Multi Camera adapter and external trigger (one source for both cameras, Trigger set to External on both cameras), the camera referring to channel 0 stops acquiring within 0 to some thousand images) I don't get any debugging error message with the new adapter. With the old adapter i get for example: "GetNumberNewImages error: first: 3473 last: 3473" for the camera on channel 0 before it stops working. The goal would be to acquire about 200,000 time points with external trigger at 20ms exposure time. I tried to understand the differences in the code from commits on 14.April, but I didn't figure out why this makes a difference for the popNextTaggedImage() function. Also: the error message "GetNumberNewImages error:" doesn't make any sense to me, referring to the situation that no new images are acquired. I would be very happy to get assistance on this one. Software: AndorDriverPack2 - 2.103.30031.0 Micro-Manager 2.0.0-gamma1 20200222 mmgr_dal_Andor.dll from 1.04.2019, 16.04.2019 and 22.02.2019 (tried many many more) best Lukas -- ____________________________________________ Lukas Hille, M.Sc. microscopy engineer Luk...@im... M +43 660 349 169 3 Department of Neurobiology, University of Vienna Campus-Vienna-Biocenter 1 1030 Vienna AUSTRIA https://neuro.univie.ac.at/ https://www.imp.ac.at/groups/manuel-zimmer/ Part of Vienna BioCenter www.viennabiocenter.org ____________________________________________ |