Re: [micro-manager-general] pixel calibration failure

[Nico S. <nic...@uc...>]

Hi Michael,

> I am struggling with the automatic pixel calibration in micro-manager 
> 2.0. I am using an Olympus IX-81 with an Orca ER camera and a Prior 
> stage.  When I run the automatic calibration I get two error messages: 
> “Error in device “Manual Focus”: Serial timout occurred.” And “XY 
> safety limit reached.” Any ideas?
>

Not sure about "Manual Focus".  It really depends which device that 
refers to (your configuration file will tell you).  The name suggests a 
Z-drive, but it is a bit odd that the pixel calibrator would move a 
Z-Drive (then again, it all depends on your configuration).  If nothing 
makes sense, use Help > Report a Problem, send the Trouble Report and 
I'll take a look.

As for "XY safety limit reached": either you are using a low (~20x or 
lower) objective and need to select the larger allowed range in the 
dialog, or the code could not reliably find the object that you placed 
in the center.  That latter problem can be painful.  If you are using 
transmitted light, try with something fluorescent (or the other way 
around).  I have worked in the past on code to make this more reliable, 
but it turned out quite hard to make this work for all kinds of 
different images.

Best,

Nico