[micro-manager-general] Troubleshooting LeicaDMI 6000B shutter behavior for multidimensional acquisition

[Aofei L. <liu...@st...>]

Hi all,

Our lab has been trying to set up micro-manager with one of our LeicaDMI
6000B microscopes (equipped with a pco.edge sCMOS camera, a Lumencor Sola
SE2 lightbox and a Prior XY stage).

We've managed to get the software to run smoothly with the microscope for
taking single snapshots and single-channel multi-timepoint acquisitions,
but we are running into some problems with multi-channel acquisition. When
more than one channel is selected, the shutter stays open after the last
channel in between time points (e.g. if we selected both TXR and GFP for
acquisition every 10 seconds, the software will take a TXR image followed
by a GFP image, then the filter cube will shift back to TXR and the shutter
will stay open for the 10 second interval until the next image, so the
sample is illuminated with green light for the entire duration).

We've tried to play around with the group settings for our channels, but
nothing seems to have fixed the problem so far. We've found some similar
queries on online forums but none of them had a resolution. Attached is our
current config file, do you have any recommendations?

Best,
Aofei