Re: [micro-manager-general] Axiovert 200M Stage Calibration and MM

[Stuurman, N. <Nic...@uc...>]

Hi Peter,

I am trying to use MM with an Axiovert 200M  and via MCU28 for stage control

All is actually going okay, regarding interface control, channel, motorised nosepiece etc;   the Pixels are calibrated and using the live image and graticule,   measurements of image, spot on.     I can also move the stage with the Device Control>Stage,  and it evens says the correct field width in um in the “Field” window

BUT, when I actually come to move the stage a set distance (or a field),  (or try to use HCS to take eg 96 wells) this is not working for stage movement.  So the actual image dimensions are okay, but it seems to send wrong pulses to MCU28 to move the desired amount

This precise problem (I think identical, as if hard wired via MM and MCU28) was in a post and reply from Nico,  but I cant see if it was solved
https://sourceforge.net/p/micro-manager/mailman/message/32075264/<https://urldefense.proofpoint.com/v2/url?u=https-3A__sourceforge.net_p_micro-2Dmanager_mailman_message_32075264_&d=DwMFAw&c=iORugZls2LlYyCAZRB3XLg&r=UwP8SWqih8VHO1LwZpgcx83I4o21yLj6V6QD-25Dt4I&m=AR_Ql4qqPGJrQnhgv7LKit79qRhCh-GN_qdQk_X6FSo&s=fA5w97T8NCEOBkWW3PoZGRIMWmEKsMt1KPpQoBRE9r4&e=>

I checked the source code and there now indeed is a pre-initialization property for this.  You will need to figure out what the real step size is (probably best to tell the stage to move a certain distance while looking at a graticule to measure how far it actually moved, this will not tell you the real step size, but give you the ratio with respect to what it is set at now).  Then go into the hardware configuration wizard, select the MCU28, and look for a (pre-initialization) property called ""StepSize(um)".  It is likely set to 0.2 microns.  Multiply this by the factor you found, save the config file, and check whether things are now moving the correct distance.  You can also edit our config file in a text editor, and change this number manually (make a copy of the file before, in case you end up making mistakes).

Best,


Nico