[micro-manager-general] HCS plugin

[PEARSON M. <mat...@ig...>]

Hi all,

I was wondering how many of you use the High Content Screening plugin within Micro-Manager?  And whether you can auto-focus reliably? I have a plastic 96 well plate and struggling to achieve consistent focus using either the 3 point auto focus or JAF (H & P) methods.  There seems to be considerable Z offset between the wells and the multi-well plate insert and stage has no grub screws for adjusting any tilt.  I am imaging at 10x so perhaps at the limit of what the 3 point interpolation focus can deal with.  I have tested JAF (H&P) be defocussing the live image and trying different z steps and i have been able to get it to work sometimes like this but as soon as i set it to capture all the imaging sites, many are out of focus.  We may try using a glass bottomed plate which should at least decrease the error inherent in the plate itself.  I just wondered how much this is being used by the community for HCS.

Thanks,

Matt


--
Matt Pearson
Microscopy Facility
MRC Human Genetics Unit
IGMM
University of Edinburgh
Crewe Road
EH4 2XU





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