Re: [micro-manager-general] Questions on Hamamatsu Flash4

[julou <tho...@no...>]

Hi Mark,

>> This is very puzzling because when we click as fast as possible on the “Add 
>> to Album” button of the GUI, we reach almost 10Hz!! So somehow MM And the 
>> camera can talk faster to each other…
> 
> Note the difference between "Snap to Album" (in the main window) and 
> "Add to Album" (in the Snap/Live window). The latter simply copies the 
> existing image to the album. 

Thanks for pointing this, I feel really stupid now…

>> Also we were able to test that this doesn’t come from our old firmware 
>> (2.03A) since Andreas Durandi (from Hamamatsu Switzerland) was kind enough 
>> to configure another flash4 (firmware 2.50A) with MM1.4 and run the same 
>> script: he obtains similar delays (≈350ms). 
> 
> These delays don't strike me as out of the ordinary. 

OK… Guillaume Witz from our lab will try to give more details on this very soon.

> The only way to (appear to) capture individual frames faster that I 
> can think of is to have a sequence acquisition running in the 
> background. In this case, snapImage()/getImage() will return the 
> latest frame from the sequence acquisition (at least for some 
> cameras). I wouldn't depend on this behavior without testing with your 
> particular camera that it returns an image acquired at the timing you 
> expect, though, since this is an area where µManager's device 
> interface is not as strictly defined as it ideally would be. 

Interesting…
Here is a variation on the same topic: provided that we manage to speed up the single-image capture to e.g. <100ms, do I understand correctly that if delay>exposure, the MDA will use single-image capture and hardware triggering for sequencable properties? then we could use the camera/MM communication time to move the stage (e.g. by triggering it with the falling edge of the camera trigger)… does this make sense?

Best,
Thomas



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