Re: [micro-manager-general] Questions on Hamamatsu Flash4

[Mark T. <ma...@op...>]

Hi Thomas,

On Tue, Dec 29, 2015 at 5:57 AM, julou <tho...@no...> wrote:
> Regarding the fast acquisition of stacks (time- or z-), I'm not clear yet on
> what is the alternative to taking a single snapshot for each point… I guess
> it's a sort of streaming mode (probably what is used in live mode as well).

Yes, it is a mode in which the frame timings are determined
autonomously by the camera (microsecond or better accuracy). Variously
called streaming, sequence acquisition, burst acquisition, etc.

> Do I understand correctly that it is used transparently in any MDA as soon
> as the exposure is longer than the delay?

Yes, that's correct.

> Does this mean that the only way for us to speed up z-stacks acquisition
> would be to use a triggerable z-stage?

Yes -- for the foreseeable future.

> Also, a new member of our group used to be able to acquire stacks faster
> (using another Flash4, maybe with more recent firmware…). He's using MM from
> Matlab (with snapImage() and getImage()) and is currently experiencing the
> same delays described in my previous email (while he used to be able to
> acquire 30ms images every 100ms, and move the stage between 2 images).
>
> Is there any setting that we could have messed up in our  config file

I can't think of anything, other than difference in the camera
version/firmware/settings.

Best,
Mark