Re: [micro-manager-general] Questions on Hamamatsu Flash4

[julou <tho...@no...>]

Thanks Nico and Mark!

Regarding the scan mode, I got an answer form Hamamatsu: slow scan is
available only with firmware ≥ 2.5.

Regarding the fast acquisition of stacks (time- or z-), I'm not clear yet on
what is the alternative to taking a single snapshot for each point… I guess
it's a sort of streaming mode (probably what is used in live mode as well).
Do I understand correctly that it is used transparently in any MDA as soon
as the exposure is longer than the delay?
Does this mean that the only way for us to speed up z-stacks acquisition
would be to use a triggerable z-stage?

Also, a new member of our group used to be able to acquire stacks faster
(using another Flash4, maybe with more recent firmware…). He's using MM from
Matlab (with snapImage() and getImage()) and is currently experiencing the
same delays described in my previous email (while he used to be able to
acquire 30ms images every 100ms, and move the stage between 2 images).

Is there any setting that we could have messed up in our  config file
<https://github.com/vanNimwegenLab/MiM_NikonTi/blob/master/Config/MMConfig_Ti_vng.cfg>  
and that would produce the delay?

Best, Thomas



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