Re: [micro-manager-general] HCS plugin- Marzhauser stage incorrect behavior

[Nico S. <nic...@uc...>]

Hi Ron,

> I am trying to use the HCS site generator to acquire data from multiwell plates and a Marzhauser stage with a PCI Tango problem.
> 
> I mark which well is A1 (upper left of plate), and then, if I try to move elsewhere on the plate, the stage moves in the X and Y axes).
> 
> When I move the stage with the mouse within MM, the directionality is ***correct***.
> 
> Is there some parameter in the plugin I can change to get the directions reversed?

Micro-Manager expects stage to move a well plate from A1 to H12 when moving into the positive direction of the X and Y axis (see: https://micro-manager.org/wiki/Coordinates_and_Directionality).  If this is not the case, then something in the stage controller or stage device adapter should take care of that change in directionality.  

I don’t have a PCI Tango and stage here, so can not experiment, but the device adapter has properties for the X and Y step size.  You could try changing the sign on them (i.e., make them negative), and see if that gives you the correct directionality.  if that works, the mouse will likely move the stage in the wrong direction.  You can fix that by changing the values for  the camera properties TransposeMirrorX and transposeMirrorY.

Hope this helps!

Best,

Nico



> 
>  
> 
> Thanks,
> 
>  
> 
> -Ron
> 
>  
> 
> Ron Goldstein, PhD
> 
> Bar-Ilan University
> 
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