Re: [micro-manager-general] Uniblitz shutter

[Nico S. <ni...@cm...>]

Hi David,

> I will look into the Thorlabs again.  I just found out that the  
> Excite is $10,500 with two LED's.  That is not going to be possible  
> at all.

> Back to the shutter- with the added file, I now get into the  
> manager adn it sees the serial adapter.  It is not clear what to  
> check in the box because it seems to show the Keyspan in one  
> checkbox and then the two ports also as separate boxes.  It allowed  
> me to proceed without error with the Keyspan checked but not the  
> other two.  It now shows the shutter, but it does not open and  
> close it. On our D1 controller, we have a local/remote switch which  
> must be in remote to allow RS232

The Keyspan driver creates two virtual serial ports that actually  
point to one and the same physical device.  One my machine they are  
called:
/dev/tty.KeySerial11
/dev/tty.USA19H3b1P1.1
You should check one of these, not both.  Best to use /dev/ 
tty.KeySerial1, since this one will be called the same even if you  
plug it into another USB port.
You will need to set the serial port to 300 baud ( and you can lower  
the 'TransmitbetweenCharsMs' to 0).  Other defaults for the serial  
port should work. Note tha you need to 'tab' out of the last property  
you edited or it will not be remembered (guess that is a bug;)

> control.  However the D122 controller has no such switch.  I am  
> trying to find out from Vincent how this is supposed to work with  
> the D122.  ANy ideas?

Did you use the Vincent D-1 controller (page 3)?
Did you 'assign' the KeySerial1 serial port to the Vincent Controller  
(page 4) by selecting it from the dropdown box?
You can leave address at 'x' (this is used when you are daisy  
chaining multiple controller), other settings should be fine (page 4).

If you are still having problems, send me (by private email) a copy  
of your config file and CoreLog.txt.

> As to the crashes, now that the Uniblitz is setup, I can't get it  
> to crash again even with all the files back in their original  
> location.  Going back to the Original configuration running the  
> firewire, it is the PVCAM driver that crashes it.  It works fine  
> with everything else in place except that one.  Dave

Thanks for checking this out!  I'll see what I can do about it.

Nico

>
> On Dec 19, 2007, at 11:19 AM, Nico Stuurman wrote:
>
>> Hi David,
>>
>>> Hi Nico- We got our NSF grant and now thinking about  
>>> implementation and purchasing.  Intergration with
>>
>> Congratulation!  It looks like we got ours too.
>>
>>> micro-manager is a key, so that is why I am playing with  
>>> shutters.  However, I really like the idea of an LED for a lab  
>>> course for safety/intensity control/longevity.  I am not  
>>> convinced I want to go the home built route because part of the  
>>> goal here is to put something together that others can easily  
>>> duplicate.  I have no sense of how hard it will be for my shop or  
>>> others to do this.  The only commercial systems I know of are the  
>>> Colibri, PrecisExcite and Fraen.  The problem is they are all  
>>> more expensive than I would like for this budget.  They do remove  
>>> the cost of a shutter and an excitation filter, but still it is  
>>> going to be over $6000 per unit.  Do you know if you can control  
>>> the PrecisExcite or Fraen LED units to be able to pick excitation  
>>> LED or power from micro-manager or how hard it would be to  
>>> implement?  Dave
>>
>> One option is the LED from Thorlabs.  I remember that it costs  
>> only $350 or so.  It would alos need a power supply.  The downfall  
>> is that it is a single color, but you can buy multiple colors and  
>> exchange manually.  Cost savings is significant.
>>
>> Also, you will still need excitation filters with an LED.  LEDs  
>> have pretty broad spectra and will give significant background  
>> without a filter.
>>
>> We currently do not directly support any of the commercial LED  
>> units, but I ma sure that it will be easy to implement once we  
>> have one of these units here in the lab for a week or so.
>>
>> Best,
>>
>> Nico
>>
>>
>>>
>>> On Dec 18, 2007, at 7:24 PM, Nico Stuurman wrote:
>>>
>>>> Hi David,
>>>>
>>>>> What options are there for controlling a Uniblitz shutter  
>>>>> through micro-manager on a Mac?  I am presuming that I would  
>>>>> run a serial cable from the Uniblitz controller to a Keyspan  
>>>>> adapter and that to the USB port?  Are there other ways of  
>>>>> doing this?  Where can I find that cable type?  Dave
>>>>
>>>> Uniblitz shutters can be used through many different  
>>>> controllers.  The Vincent controllers all use a serial  
>>>> interface.  You will indeed need a USB-serial adapter (like the  
>>>> ones from Keyspan) to connect these to your Mac.
>>>>
>>>>> PS- It would be nice if this info were part of the Uniblitz  
>>>>> help page in the documentation
>>>>
>>>> To this purpose I moved all device info to a Wiki (https:// 
>>>> valelab.ucsf.edu/~nico/MMwiki).  The page on the Vincent  
>>>> controllers can be found here: https://valelab.ucsf.edu/~nico/ 
>>>> MMwiki/index.php/Vincent.
>>>> Please help me by editing the pages (you will need to make an  
>>>> account) with information that you found to be relevant and  
>>>> missing.
>>>>
>>>> Best regards,
>>>>
>>>> Nico
>>>>
>>>>
>>>>
>>>>>
>>>>> Dr. David Knecht
>>>>> Department of Molecular and Cell Biology
>>>>> Co-head Flow Cytometry and Confocal Microscopy Facility
>>>>> U-3125
>>>>> 91 N. Eagleville Rd.
>>>>> University of Connecticut
>>>>> Storrs, CT 06269
>>>>> 860-486-2200
>>>>> 860-486-4331 (fax)
>>>>>
>>>>>
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>>>
>>> Dr. David Knecht
>>> Department of Molecular and Cell Biology
>>> Co-head Flow Cytometry and Confocal Microscopy Facility
>>> U-3125
>>> 91 N. Eagleville Rd.
>>> University of Connecticut
>>> Storrs, CT 06269
>>> 860-486-2200
>>> 860-486-4331 (fax)
>>>
>>>
>>
>
> Dr. David Knecht
> Department of Molecular and Cell Biology
> Co-head Flow Cytometry and Confocal Microscopy Facility
> U-3125
> 91 N. Eagleville Rd.
> University of Connecticut
> Storrs, CT 06269
> 860-486-2200
> 860-486-4331 (fax)
>
>