I am trying to analyze an SEM .tif file obtained from HITACHI TM3000 Tabletop Scanning Electron Microscope, but having trouble setting the sale. Could anyone please help me understand how to set it?
Thanks.
The accompanied SEM parameter file is attached below -
Can you please attach both the txt header and the image file here (using Add attachments)?
This looks like a data format that should be loaded automatically by Gwyddion with correct scale. But I cannot check what really happens just from the header pasted to the question.
If you would like to refer to this comment somewhere else in this project, copy and paste the following link:
The file loads fine for me. You probably just need to open the header in Gwyddion, not the TIFF image. This is generally the preferred way of opening data split to header and raw image data files.
The reason is that Gwyddion can recognise the header files, the headers say how to find the associated data files, etc. Going back from the image data to the header is not so easy because Gwyddion opens files in 120+ different formats (a bunch of them TIFF-based) and it does not know that in this specific case it should look for an associated .txt file.
If you would like to refer to this comment somewhere else in this project, copy and paste the following link:
I drag & dropped the header file and it opened fine. But what would be the units for roughness parameters? There is no units shown. Also, how do i tell Gwyddion the depth of the wire?
If you would like to refer to this comment somewhere else in this project, copy and paste the following link:
An SEM image contains recorded brigthness in pixels, similar to a photograph, just using electrons. SEM does not directly measure topography (like AFM) and the reconstruction of the 3D shape from a SEM picture is as difficult as from a photograph. There is specialised SEM software that performs (or at least attempts) such reconstruction, either from mutliple images taken from different angles using epipolar geometry, or just from shading (more like a gimmick this one). From the metrological point of view the results are questionable at best but can be still of some value if no better method is available. In any case, Gwyddion does implement this reconstruction (we focus on AFM where topography is actually directly measured). The values you see are brightness values. This is what the SEM measured -- and this is also what the statistical quantities tool evaluates. Hence only lateral measurements in the image are meaningful. There is no topographical information.
If you would like to refer to this comment somewhere else in this project, copy and paste the following link:
I am trying to analyze an SEM .tif file obtained from HITACHI TM3000 Tabletop Scanning Electron Microscope, but having trouble setting the sale. Could anyone please help me understand how to set it?
Thanks.
The accompanied SEM parameter file is attached below -
[SemImageFile]
Version=02-01
InstructName=TM3000
SerialNumber=104598-07
DataNumber=0046
SampleName=
Format=TIF
ImageName=BCCZ-4.tif
Directory=C:\Users\User\Favorites\Desktop\tone_n_tune\ SaveMode=2
Date=16/08/2016
Time=18:56:58
DateCheck=No
TimeCheck=No
NumberCheck=No
CommentCheck=No
DigitalZoom=No
Media=DriveC
DataSize=1280x1100
DPI=200.69
PixelSize=506.25
SignalName=BSE COMPO
AcceleratingVoltage=15000 Volt
DecelerationVoltage=
Magnification=250
WorkingDistance=6900 um
EmissionCurrent=72500 nA
FilamentCurrent=1750 mA
ObservationCondition=Normal
Brightness=2223
Contrast=1411
Rotation=0
LensMode=
PhotoSize=
Vacuum=High
MicronMarker=300000
SubMagnification=
SubSignalName=
SpecimenBias=
Condencer2=2767
ScanSpeed=Slow3
CalibrationScanSpeed=
ColorMode=Grayscale
ColorPalette=
ScreenMode=
Comment=[]
KeyWord1=
KeyWord2=
Condition=Vacc=15.0kV Mag=x250 WD=6.90mm
DataDisplayCombine=
StageType=0
StagePositionX=0
StagePositionY=0
StagePositionR=
StagePositionZ=
StagePositionT=
Last edit: tone_n_tune 2016-12-11
Can you please attach both the txt header and the image file here (using Add attachments)?
This looks like a data format that should be loaded automatically by Gwyddion with correct scale. But I cannot check what really happens just from the header pasted to the question.
Hi David, I have attached both the files, thanks for having a look.
Edit: Files removed as they are part of a research project.
Last edit: tone_n_tune 2016-12-11
The file loads fine for me. You probably just need to open the header in Gwyddion, not the TIFF image. This is generally the preferred way of opening data split to header and raw image data files.
The reason is that Gwyddion can recognise the header files, the headers say how to find the associated data files, etc. Going back from the image data to the header is not so easy because Gwyddion opens files in 120+ different formats (a bunch of them TIFF-based) and it does not know that in this specific case it should look for an associated .txt file.
I drag & dropped the header file and it opened fine. But what would be the units for roughness parameters? There is no units shown. Also, how do i tell Gwyddion the depth of the wire?
An SEM image contains recorded brigthness in pixels, similar to a photograph, just using electrons. SEM does not directly measure topography (like AFM) and the reconstruction of the 3D shape from a SEM picture is as difficult as from a photograph. There is specialised SEM software that performs (or at least attempts) such reconstruction, either from mutliple images taken from different angles using epipolar geometry, or just from shading (more like a gimmick this one). From the metrological point of view the results are questionable at best but can be still of some value if no better method is available. In any case, Gwyddion does implement this reconstruction (we focus on AFM where topography is actually directly measured). The values you see are brightness values. This is what the SEM measured -- and this is also what the statistical quantities tool evaluates. Hence only lateral measurements in the image are meaningful. There is no topographical information.
That was a good explanation. Any suggestion on how to represent the roughness parameters in this case?