DownloadF-Tracker3D is acronym of "fluorescent cell tracker in three-dimensions". It is a software tool designed to track fluorescent cells using time-lapse z-stacks of images acquired with a confocal or light-sheet microscope. First, each single cell is tracked in 2D by using the maximum intensity projections of the z-stacks and CellTracker, the free open source software available at: www.celltracker.website. Then, the 3D track of each cell is obtained analysing the z-stacks and looking for the z-plane with the maximum intensity value for the different x-y positions previously determined.
F-Tracker3D is written in MATLAB (©, The MathWorks, Inc., Massachusetts, USA) and the source code is freely provided.
Requirements: MATLAB 2015a and Image Processing Toolbox 9.2 or later versions.
Features
- Matlab standalone application
- 3D tracking
- CellTracker
- Fluorescent cells
- Confocal microscope
- Light-sheet microscope
Project Samples
