Often when I combine multiple sequences from the data pool into a single ring, it will show up blank. The space is still there, but there is no data displayed on the ring. BRIG can usually handle 2 genomes, but often when I add a third, the ring becomes blank.
If I go back and delete the sequence that caused it to turn blank, it will usually still be blank on rerun. I can reduce it all the way to just one sequence, and it still shows up blank. It seems the only way to get rid of this is to close out the program and restart.
It's an irregular problem, however. I have combined as many as 11 genomes into a single ring before. But then when I add ≥ 3 sequences to a different ring, that one shows up blank; the ring with 11 sequences is still fine.
In the attached jpg, Ring 1 has one sequence, Ring 2 has 3 sequences, and Ring 3 has 11 sequences. I ran this before with Ring 2 = 2 sequences and it was fine.
Ring 2 disappeared when I added a third sequence