When running Bowtie2 (versions 2.2.2) I get this error (using this command line option: --un-conc '/projects/panel/output/Vvvv-mmm-hhhh-7.1.6uM__1/rrrrr-ffllelll_ttttt/'):
(ERR): Could not open --un-conc mate-1 output file '/projects/panel/output/Vvvv-mmm-hhhh-7.1.6uM__1/rrrrr-ffllelll_ttttt/' at /tools/bowtie2/bowtie2 line 402.
Exiting now ...
BUT if one changes this:
--un-conc '/projects/panel/output/Vvvv-mmm-hhhh-7.1.6uM__1/rrrrr-ffllelll_ttttt/'
into this:
--un-conc '/projects/panel/output/ttttt/'
then Bowtie2 will run just fine (while keeping all the rest of options exactly the same)!
It looks like this error appears if there is a dot in the path name given to "--un-conc"!
Hi Daniel,
Thank you for letting us know about this problem. A fix will be available for the next release. Until then a fix proposal can be download it from here: https://github.com/BenLangmead/bowtie2/archive/bugs/313.zip
thanks,
Val
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