Showing 70 open source projects for "pcr"

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    Nitro Secure Module library

    Nitro Secure Module library

    Provides Nitro Enclaves with attestation capability

    ...This is a collection of helpers which Nitro Enclaves userland applications can use to communicate with a connected NitroSecureModule (NSM) device. Various operations can be requested such as PCR query and manipulation, attestation, entropy.
    Downloads: 0 This Week
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    pydna

    pydna

    Clone with Python! Data structures for double stranded DNA

    Clone with Python! Data structures for double stranded DNA & simulation of homologous recombination, Gibson assembly, cut & paste cloning. Planning genetic constructs with many parts and assembly steps, such as recombinant metabolic pathways, are often difficult to properly document as is evident from the poor state of documentation in the scientific literature. The pydna python package provide a human-readable formal description of cloning and genetic assembly strategies in Python which...
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  • 3
    Gemi

    Gemi

    PCR primers / probes design from multiple & degenerate sequences

    Gemi, an automated, fast, and easy-to-use bioinformatics tool with a user-friendly interface to design primers and probes for polymerase chain reaction (PCR). Gemi accepts multiple aligned and long DNA and RNA sequences with degenerate nucleotide (non-A/C/G/T bases). Gemi can be used for quantitative, real-time and conventional PCR (qPCR, rt-PCR, etc.), and Sanger sequencing. Gemi can parse large dataset of sequences efficiently. Python source code is available upon request. Milestone: The tool reached about 3000 downloads sine 2012. ...
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  • 4
    CCE5

    CCE5

    Plataforma de Control de Calidad

    Plataforma para el Control de Calidad de Diagnóstico en Serología y PCR para la enfermedad de Chagas
    Downloads: 0 This Week
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  • 5

    DVB Inspector

    DVB transport stream analyzer written in java

    DVB Inspector is a java program to analyze captured DVB-streams. It is not a real time tool that can interact with PC-TV DVB hardware directly. You have to use another program to record a Transport stream. It can show the logical structure of the DVB SI and PSI data. It can also show bit rate usage data. Support for for DSM-CC Object carousels, teletext, DVB subtitles Version control has moved to https://github.com/EricBerendsen/dvbinspector . New versions will be available for download...
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    Downloads: 17 This Week
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  • 6

    FullSSR

    SSR detection and primer designing software

    ...Nowadays is easier and cheaper to obtain genomic data from next generation sequencing. Then, at least two software need to be applied in order to obtain SSR primers of SSR flanking sequences for PCR assays. Here we propose new software that allows the user to work with several sequences for PCR primer design in one step.
    Downloads: 1 This Week
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  • 7
    covid19

    covid19

    Tokyo COVID-19 Task Force website

    New coronavirus infectious disease control site. In Tokyo, in order to centrally announce the infection status in Tokyo, we received reports of outbreaks not only in the jurisdiction of the Tokyo Health Center but also in the cities with health centers (Machida City, Hachioji City) and 23 wards, and aggregated these. In Tokyo, we have set seven monitoring items for the new coronavirus infection, with the two pillars of "infection status" and "medical care provision system" in Tokyo. Every...
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  • 8
    Downloads: 0 This Week
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  • 9
    Downloads: 0 This Week
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  • 10
    Downloads: 0 This Week
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  • 11
    Design PCR primers from DNA sequence. Widely used (190k Google hits for "primer3"). From mispriming libraries to sequence quality data to the generation of internal oligos, primer3 does it. C&perl. Developers/testers/documenters needed.
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    Downloads: 154 This Week
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  • 12

    GMATA software for Genomic SSR marker

    Genome-wide Microsatellite Analyzing Toward Application: GMATA

    What is software GMATA v21 Genome-wide Microsatellite Analyzing Toward Application (GMATA) is a software for Simple Sequence Repeats (SSR) analyses, and SSR marker designing and mapping in any DNA sequences. It has the following functions: 1. SSR mining; 2. Statistical analysis and plotting; 3. SSR loci graphic viewing; 4. Marker designing; 5. Electronic mapping and marker transferability investigation. GMATA is accurate, sensitive and fast. It was designed to process large genomic...
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    Downloads: 4 This Week
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  • 13

    WellMeth

    Genome-Wide DNA Methylation Analysis with RRBS

    WellMeth is a integrated framework for Reduced Representation Bisulfite-Seq analysis
    Downloads: 0 This Week
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  • 14

    Qiagen / Roche converter

    Converts Qiagen XML-files to Roche Light CSV-Files.

    ...The converts the Qiagen XML file including the position and sample number to a txt file which than can be importet into the Sample Sheet of the Roche LightCycler/Cobas 480 Software. It's very convinient if you are working with microtitier plates and high-throughput Coronavirus-PCR. No instalation required. Just drop you XML file into the folder "in_qiagen" and execute the qiagen_roche.exe file. The resulting Roche-file can be found in "out_lc480". *Notice that the folder "in_qiagen" and "out_lc480" must be in the same directory as the qiagen_roche.exe file The qiagen_roche.c contains the sourcecode of the program. ...
    Downloads: 0 This Week
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  • 15
    The SPInDel workbench is a computational platform to facilitate the planning and management of SPInDel projects, alignment of nucleotide sequences, visualization and selection of conserved regions, calculation of PCR primers properties, prediction of SPInDel profiles and diverse statistical and phylogenetic analyses. It includes a large dataset comprising nearly 1,800 numeric profiles for the identification of eukaryotic, prokaryotic and viral species.
    Downloads: 0 This Week
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  • 16
    RDML stands for Real-time PCR Data Markup Language and is the standard file format for export, storage and exchange of quantitative PCR (qPCR) data. This project focuses on the development, implementation and enhancement of the RDML file format and its description (XML schema).
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  • 17

    GM calculator

    for a geometric mean size of a given particle size distribution

    ...For more detail, please see and cite our following paper: Yamamoto, N., Nazaroff, W.W., Peccia, J. (2014). Assessing the aerodynamic diameters of taxon-specific fungal bioaerosols by quantitative PCR and next-generation DNA sequencing, Journal of Aerosol Science 78, 1-10.
    Downloads: 0 This Week
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  • 18

    PrimerSeq

    Designs primers on flanking constitutive exons for RT-PCR

    Downloads: 0 This Week
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  • 19

    MPprimer

    a program for reliable multiplex PCR primer design

    Background: Multiplex PCR, defined as the simultaneous amplification of multiple regions of a DNA template using more than one primer set (comprising a forward primer and a reverse primer) in one tube, has been widely used in clinical and environmental microbiology studies. However, primer design for multiplex PCR is still a challenging problem and several factors need to be considered.
    Downloads: 3 This Week
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  • 20

    UMI-Reducer

    collapsing technical duplicates via Unique Molecular Identifiers

    Every sequencing library contains duplicate reads. While many duplicates arise during polymerase chain reaction (PCR), some of these duplicates derive from multiple identical fragments of mRNA present in the original lysate (termed "biological duplicates"). Unique Molecular Identifiers (UMIs) are random oligonucleotide sequences that allow differentiation between technical and biological duplicates. Here we report the development of UMI-Reducer, a new computational tool for processing and differentiating PCR duplicates from biological duplicates. ...
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  • 21

    MT-Toolbox

    Software for molecule tagged DNA sequences

    MT-Toolbox is a user friendly software package for analysis of molecule tagged Illumina MiSeq reads. Molecule tagging is a molecular biology technique to significantly reduce amplicon sequence error and PCR bias which can be applied to any amplicon sequencing project. MT-Toolbox converts raw reads into high quality consensus sequences based on each reads molecule tag. For details and other important information please refer to the MT-Toolbox webpage below. MT-Toolbox is now hosted on github here: https://github.com/islandhopper81/mt-toolbox
    Downloads: 0 This Week
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  • 22

    Edesign

    Primer and enhanced internal probe design tool

    Edesign is a design tool for PCR primers together with an internal probe for conducting quantitative PCR and genotypic experiments. - Functions for designing internal probes are greatly enhanced. - "Genotyping mode" provides design of the internal probe over a user-specified target variant e.g. (SNP, insert/deletion, somatic mutation etc) for genotyping. - Modified oligonucleotide can be designed.
    Downloads: 0 This Week
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  • 23

    MSP-HTPrimer

    A high-throughput primer design tool for DNA methylation analysis

    MSP-HTPrimer is an open source, web-based high-throughput and genome-wide primer design pipeline for bisulfite-based assays (MSP, BSP and COBRA) and MSRE-PCR assay and capable of simultaneously processing hundreds to thousands of target sequences. MSP-HTPrimer takes genome-wide annotations of SNPs and repeats into consideration to design primer pairs for higher success rate. MSP-HTPrimer enables hierarchical filtering and visualisation of designed primers in UCSC genome browser for efficient selection of assays.
    Downloads: 1 This Week
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  • 24

    sgRNAcas9

    a software for designing CRISPR sgRNA and evaluating Off-Target Sites

    This package consists of programs to perform a search for CRISPR target sites (protospacers) with user-defined parameters, predict genome-wide Cas9 potential off-target cleavage sites (POT), classify the POT into three categories, batch design oligonucleotides for constructing 20-nt (nucleotides) or truncated sgRNA expression vectors, extract desired length nucleotide sequences flanking the on- or off-target cleavage sites for designing PCR primer pairs to validate the mutations by T7E1 cleavage assay. Importantly, by identifying potential off-target sites in silico, the sgRNAcas9 allows the selection of more specific target sites and aids the identification of bona fide off-target sites, significantly facilitating the design of sgRNA for genome editing applications. ...
    Downloads: 2 This Week
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  • 25

    MSRE-HTPrimer

    A high-throughput primer design tool for MSRE-assay in epigenetics

    ...MSRE-HTPrimer provides significant improvements over existing solutions with following unique features: 1) visualization of primer pairs in UCSC genome browser, 2) search each resulting primer pair in UCSC In-Silico PCR database, 3) flexible primer selection and filtering based on custom quality matrix and 4) parallel primer design for several target sequences. The pipeline is equipped with multiprocessing capability and uses custom inputs and parameters to design specific primers.
    Downloads: 2 This Week
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