GetX
An automated mutation cassette design tool for scarless genome editing
..., insertions, and point mutations. It also designs synonymous codon fragments required to manipulate essential target genes to enable survival of the strain during the genome editing procedure. GetX provides genbank and fasta format files for a mutation template plasmid, a mutation cassette, and a sequence around the mutation site to guide the user through the design of the materials required for the scarless bacterial genome editing method.