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cross_genome.README 2014-08-22 786 Bytes
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#Cross_genome: genome scaffolding using cross-species synteny
# Or merge scaffolds from two assembly files: ref.fastq assembly.fastq

/tmp/src/cross_genome -gap 20000 -nodes 20 -score 100 <ref_fasta/q_file> <assembly_fasta/q_file> <merged.fasta_file>

/tmp/src/cross_genome -gap 20000 -nodes 20 -score 100 ref_fasta target_fasta merged.fasta

gap:   maximum gap size allowed to join the two contigs
node:  number of CPU threads
score: Smith-waterman score


Note: 

(1) You need to type the full path in order to make it work;
(2) Using larger gap size can increase scaffold size, but sometimes, it
also brings errors.

========
Install:
========
gunzip cross_genome.tar.gz
tar xvf cross_genome.tar
make


Please contact Zemin Ning ( zn1@sanger.ac.uk ) for any further information.

 

Source: cross_genome.README, updated 2014-08-22