From: Thomas E. <te...@mb...> - 2010-01-02 01:01:21
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Jason, When I use optAlign I get the same value as with ProFit (4.615214 A.), that's why I suspected that 'align' omits some distant CA atoms when calculating the RMSD. In fact I just noticed that the last 2 residues are not assigned an asterisk or a dot in the alignment. So, when I exclude them from 'optAlign' I get 1.780601 A., the same value as with 'align'. Thomas > Thomas, > > If PyMOL has paired two atoms for alignment, then they will be used in > the calculation of the RMSD. > > Also, this sounds like you should be using 'fit' or 'optAlign' to be > superposing the molecules. (If you already know the atom pairings, no > 'alignment' step is necessary.) > > Possibly helpful link: > http://www.pymolwiki.org/index.php/Category:Structure_Alignment > > See: fit, optAlign, intra_fit_rms. > > Hope this helps, > > -- Jason > > -- > Jason Vertrees, PhD > > PyMOLWiki -- http://www.pymolwiki.org > > > > On Tue, Dec 29, 2009 at 1:27 PM, Thomas Evangelidis > <te...@mb...> wrote: >> Dear pymol users, >> >> I'm trying to understand how align command measures the RMSD between 2 >> homologues and explain the discrepancy between that value and the one >> obtained from ProFit. The sequence alignments produced by both >> programs are continuous and match, but the RMS values are 1.781 and >> 4.615 for Pymol and Profit respectively. The RMS corresponds to Ca >> atoms only (pymol command line: "align 3G5U_A and name CA and chain A, >> H_ABCB1_NBD1 and name CA, cycles=0, object=ao"). Does pymol consider >> only Ca atoms that are closer than a default cutoff distance only >> (e.g. 5 A) when calculating the RMSD? >> >> >> ------------------------------------------------------------------------------ >> This SF.Net email is sponsored by the Verizon Developer Community >> Take advantage of Verizon's best-in-class app development support >> A streamlined, 14 day to market process makes app distribution fast and easy >> Join now and get one step closer to millions of Verizon customers >> http://p.sf.net/sfu/verizon-dev2dev >> _______________________________________________ >> PyMOL-users mailing list (PyM...@li...) >> Info Page: https://lists.sourceforge.net/lists/listinfo/pymol-users >> Archives: http://www.mail-archive.com/pym...@li... >> > ----- End message from jas...@gm... ----- |