Re: [micro-manager-general] micro-manager on leica microscopes Vs IPP

[Nuno M. <mo...@ig...>]

Hi again,

As far as I know there is no way in IPP to set a delay for the shutter 
and it doesn't seem to be reporting full opening. The way I'm doing know 
  is to used IPP macros an then call a homemade C small program that 
sets the shutter of an EL6000 Leica Metal halide bulb this a delay 
(however there this not elegant synchronization its not precise). This 
shutter is suppose to operate in 10 ms range so I see no reason for the 
delay.

Just one more thing. As far as I know the Zeiss does not use direct 
serial commands like Nikon. Which method do you suggest, or better what 
would be the easier ;)

Regards,
NM

Nico Stuurman wrote:
> Hi Nuno,
> 
>> I just register yesterday to this mailing list. I have been  
>> browsing in
>> the archives and Ricardo (like he told me in the EMBO course, Hi
>> Ricardo) already started to make something on the driver for  
>> controlling
>> leica microscopes. As far as I know, newer stands have different
>> communication protocol, but I'm actually interested in controlling a
>> DMIRE2, the older one.
>>
>> I know that Nico also have something started, can you guys send me the
>> code so that I can continue?
> 
> I have indeed seen some emails on- and off-list from people working  
> on or planning to work on adapters for Leica microscopes.   
> Regretfully, I have not yet seen any code, so there is nothing I can  
> send you.  If you are on Windows, it is best to start with the Device  
> Developer's kit (http://www.micro-manager.org/downloads.php? 
> object=devkit) using the ZeissCAN adapter code as an example.
> 
>> My other question is: We have now imagepro plus controling an ORCA AG
>> and DMIRE2 microscope but the camera takes like 500 ms until it really
>> gets the picture. This means that the shutter stays opened 500ms+
>> exposure time which is unacceptable! Do you think that it would be
>> faster with micro-manager?
> 
> Micro-Manager waits for the shutter to be fully open before it takes  
> the image.  If you shutter is really slow (i.e., it takes 500 msec  
> for the shutter to be fully open), then the same would happen with  
> Micro-Manager.  If your shutter is much faster, Micro-Manager will be  
> faster as well.  A shutter can either report itself whether it is  
> open or closed, or you can set a 'delay' (time the program will wait  
> after opening the shutter before it starts the image acquisition).   
> To be fair, I would expect ImagePro to use the same principles. Maybe  
> you can adjust the ImagePro configuration to mitigate this problem?
> 
> Best,
> 
> Nico
> 
> 
>> Regards,
>> -- 
>> Nuno Moreno
>> Cell Imaging Unit
>> Instituto Gulbenkian de Ciência
>> http://uic.igc.gulbekian.pt
>> http://www.igc.gulbekian.pt
>> phone +351 214464606
>> fax   +351 214407970
>>
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-- 
Nuno Moreno
Cell Imaging Unit
Instituto Gulbenkian de Ciência
http://uic.igc.gulbekian.pt
http://www.igc.gulbekian.pt
phone +351 214464606
fax   +351 214407970