Dear Colleague, Many thanks for your interests in Scaff10x. Say you have two fastq files from 10x platform: read_1.fastq read_2.fastq You convert the fastq files first: /nfs/users/nfs_z/zn1/src/scaff10x/scaff_BC-reads-1 read_1.fastq read-BC_1.fastq read-BC_1.name > try.out /nfs/users/nfs_z/zn1/src/scaff10x/scaff_BC-reads-2 read-BC_1.name read_2.fastq read-BC_2.fastq > try.out Here scaff_BC-reads-1 does (1) extract the fist 16 barcode bases from read 1 and append to read names Note the read name here...