Hi dears, Actually I am trying to use your tool to calculate the allele frequency of my Whole genome data. For this porpuse, all steps were being done smoothly but for the step called "Calculate allele frequency differences", the command gets done with giving incomplete out put files. The version of the perl in our server is 5.22 but you have said it should be 5.8 or higher. Do you think is that possible to be effective? Thank you very much in advance. Regards, Omid