Elizabeth LeClair

I'm running Scalpel but getting almost immediate failure/exit from the program and I'm not sure why. Red flags are "undefined sequence" and "command failure" I'm quite new to Linux commands so I can't really tell if it's my input files or an installation problem . Here's the detailed output, can anyone assist? INPUT: $ perl /mnt/d/scalpel-0.5.4/scalpel-discovery --single --bam /mnt/d/scalpel-0.5.4/MC-files/MC-fish.marked_duplicates.bam --bed 15:1-48040578 --ref /mnt/d/scalpel-0.5.4/MC-files/danRer11.fa...

Hello, new user here. I downloaded the software packages and also the first two sample FASTQ files. But these files don't download. The error is "no such directory". Is there a solution? ERR194151_1.fastq.gz ERR194151_2.fastq.gz ERR324432_1.fastq.gz ERR324432_2.fastq.gz $ wget --no-check ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR194/ERR194151/ERR194151_1.fastq.gz --2021-01-08 10:17:53-- ftp://ftp.sra.ebi.ac.uk/vol1/fastq/ERR194/ERR194151/ERR194151_1.fastq.gz => ‘ERR194151_1.fastq.gz’ Resolving ftp.sra.ebi.ac.uk...