Analyzing poor data takes CPU time and interpreting the results from poor data takes people time, so it's always important to make a pre-processing.
Let me call my script as “Sequence_cleaner” and the big idea is to remove duplicate sequences, remove too short sequences ( the user defines the minimum length) and remove sequences which have too many unknown nucleotides (N) ( the user defines the % of N is allows ) and in the end the user can choose if he/she wants to have a file as output or print the result.
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Hi, to keep your computer performance better, delete your all duplicate file by using the utility program from DuplicateFilesDeleter.com. It works fast.