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Root Estimator for Shovelomics Traits (REST) is a software developed for an automated or semi automated high-throughput analysis of root system traits form high quality RGB pictures. Standardized image acquisition is strongly recommended and briefly described in the manual which you can get here for free http://plantscience.psu.edu/research/labs/roots/publications/overviews/next-generation-shovelomics-set-up-a-tent-and-rest
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