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We designed and implemented a pipeline for fast and accurate quantification of both known miRNAs and isomiRs by joint processing multiple samples. QuickMIRSeq reconciles its implementation with the unique nature of miRNAs.All analyses results are organized in a user friendly manner, accessible via a web interface, and can be drilled down interactively by investigators or analysts to conduct further investigation and discovery. QuickMIRSeq is a powerful and flexible open source tool for accurate quantification of known miRNAs/isomiRs.

The software package, and the test run results for miRNA-seq dataset GSE64977(human), GSE65920(mouse) and GSE60900(rat) can be downloaded directly from https://sourceforge.net/projects/quickmirseq/files/, and are also available online https://baohongz.github.io/QuickMIRSeq/

QuickMIRSeq Web Site



  • Joint mapping of multiple samples
  • Strand aware
  • Quantification of both miRNAs and isomiRs
  • Accurancy and reliablity of detected miRNAs
  • User friendly summary report


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