ProGraphMSA

ProGraphMSA is a state-of-the-art multiple sequence alignment tool which produces phylogenetically sensible gap patterns while maintaining robustness by allowing alternative splicings and errors in the branching pattern of the guide tree. This is achieved by incorporating a graph-based sequence representation as in POA and combines it with the advantages of the phylogeny-aware algorithm in Prank. Further, we account for variations in the substitution pattern by using estimated amino acid frequencies and by implementing context-specific profiles as in CS-Blast.
The latest versions of ProGraphMSA include and extension to the alignment model to support tandem repeat unit insertions and deletions.