OME-3DR

For big OME data analysis, we integrate commonly used quantitative methods, describe our novel strategies to quantify and analyze biological markers related to the cell or organelle spatial-coordinate model, and present open-source OME-3-Dimensional Reconstruction (OME-3DR), a flexible, programmable and batch-oriented tool based on OME data, for reconstructing 3-dimensional (3D) spatial conformations and conducting further analyses, such as the identification, counting, localization and tracking of bio-imaging markers, calculation of model-contour parameters for association analyses, establishing spatial-coordinate system, 3D co-localization analyses and so on. OME-3DR is well modularized (for automation, three main menus integrate hundreds of sub-functions), easy to use and ingenious; indeed, after setting the corresponding parameters, batch processing is relatively straightforward when beginning the 3D model reconstruction...