Gel Blot Analyzer is a comprehensive desktop application designed to streamline the annotation, analysis, and presentation of gel electrophoresis images. Built for researchers, students, and scientists, this tool simplifies the often tedious process of preparing gel images (SDS-PAGE, Western Blots, DNA gels, etc.) for lab notebooks, presentations, and publications.
From precise labeling and molecular weight prediction to powerful densitometric analysis, Gel Blot Analyzer combines essential imaging tools into a single, intuitive workflow.
Since this application is not signed on Windows first run, please click on Run Anyway after uncompressing. For MACOS, please uncompress and copy the DMG file to the applications folder, go to terminal and run this command:
xattr -cr "/Applications/Gel Blot Analyzer.app"
For more details and information, please visit the GitHub: https://github.com/Anindya-Karmaker/Gel-Blot-Analyzer
Features
- Broad Format Support: Works with most common image formats (TIFF, PNG, BMP, JPG) from any imaging device.
- mage Transformation: Integrated toolkit for cropping, rotation, alignment, and perspective (skew) correction.
- Advanced Adjustments: Fine-tune image levels (black/white points) and gamma to visualize faint bands without altering raw data for analysis.
- Effortless Annotation: Place standard (L/R/Top) or fully custom markers. Draw lines, arrows, and shapes with custom fonts, colors, and sizes.
- Densitometry Suite: Perform quantitative analysis by defining lanes with rectangular or quadrilateral selections to handle skewed gels.
- Peak Analysis: Features multiple background subtraction methods (Rolling Ball, Valley-to-Valley) and tunable peak detection for accurate band quantification.
- MW Prediction: Predict the molecular weight of unknown bands using a standard curve generated from your markers with multiple regression models.
- Glycan/Oligomer Analysis: Visualize and predict different protein bands based on glycosylation sites and dimer, trimer, tetramer or other oligomer configuration.
- Presets & Workflow: Save your favorite molecular weight marker layouts as presets. Undo/Redo support for a non-destructive workflow.
- Professional Output: Export your final annotated image with a transparent background, perfect for posters and publications along with detailed reports for densitometry and Microsoft Excel file for further analysis..
- Robust & Stable: includes a built-in exception logger to help diagnose any issues and ensure a stable user experience.