F-Tracker3D is acronym of "fluorescent cell tracker in three-dimensions". It is a software tool designed to track fluorescent cells using time-lapse z-stacks of images acquired with a confocal or light-sheet microscope. First, each single cell is tracked in 2D by using the maximum intensity projections of the z-stacks and CellTracker, the free open source software available at: www.celltracker.website. Then, the 3D track of each cell is obtained analysing the z-stacks and looking for the z-plane with the maximum intensity value for the different x-y positions previously determined.

F-Tracker3D is written in MATLAB (©, The MathWorks, Inc., Massachusetts, USA) and the source code is freely provided.

Requirements: MATLAB 2015a and Image Processing Toolbox 9.2 or later versions.

Features

  • Matlab standalone application
  • 3D tracking
  • CellTracker
  • Fluorescent cells
  • Confocal microscope
  • Light-sheet microscope

Project Samples

Play Video F-Tracker3D: official logo F-Tracker3D: automatic plot of the 3D tracks F-Tracker3D: time-lapse experiment with fluorescent cells floating in a 3D spheroid F-Tracker3D: main graphical user interface F-Tracker3D: statistics GUI

Project Activity

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Categories

Bio-Informatics, Image Processing

License

GNU General Public License version 3.0 (GPLv3)

Follow F-Tracker3D

F-Tracker3D Web Site

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Additional Project Details

Operating Systems

Windows

Languages

English

Intended Audience

Science/Research

Programming Language

MATLAB

Related Categories

MATLAB Bio-Informatics Software, MATLAB Image Processing Software

Registered

2016-10-06
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