easy-dhpsf

This package of MATLAB routines with accompanying GUI allows scientific users to measure the 3D location of single molecules when using the double-helix point spread function (DH-PSF) widefield microscope. Version 2 also includes code that facilitates combining localization data from two spectral channels using a locally-weighted quadratic 3D registration function

Features
- Double-Gaussian estimator is calibrated via an axial scan of bright immobile fluorescent emitters (e.g. beads)
- DH localization is performed through template matching and subsequent double Gaussian fitting
- Tiff stacks of SM images are analyzed using template matching followed by double-Gaussian fitting to extract estimates of the molecule positions
- Two spectral channels are registered by generating a locally-weighted quadratic mapping function from control point pairs

Project Activity

See All Activity >

License

BSD License

Follow easy-dhpsf

easy-dhpsf Web Site

Other Useful Business Software

Build on Google Cloud with $300 in Free Credit

New to Google Cloud? Get $300 in free credit to explore Compute Engine, BigQuery, Cloud Run, Vertex AI, and 150+ other products.

Start your next project with $300 in free Google Cloud credit. Spin up VMs, run containers, query exabytes in BigQuery, or build AI apps with Vertex AI and Gemini. Once your credits are used, keep building with 20+ products with free monthly usage, including Compute Engine, Cloud Storage, GKE, and Cloud Run functions. Sign up to start building right away.

Start Free Trial

Rate This Project

User Reviews

Be the first to post a review of easy-dhpsf!

Additional Project Details

Intended Audience

Science/Research

Programming Language

MATLAB

Related Categories

MATLAB Molecular Science Software, MATLAB Chemistry Software, MATLAB Physics Software

Registered

2015-09-05

Similar Business Software

SDS Manager

SDS Manager is a leading provider of Safety Data Sheet (SDS) management systems, offering one of the largest SDS databases with over 14 million documents available in 25 languages. With SDS Manager, employees can easily access Safety Data Sheets directly from their mobile devices by scanning...

See Software
Lockbox LIMS

A sample tracking, test result capture, and inventory management cloud LIMS for life science research, biotech/NGS, and industrial QC labs. Includes regulatory support for CLIA, HIPAA, Part 11, and ISO 17025. Nothing is more critical to a lab’s success than the quality, security, and...

See Software
QBench

The modern, flexible, easy-to-use LIMS. QBench enables our customers to get a LIMS up and running faster. Automate your entire lab with our developer-friendly API, Inventory Management, Customer Portal, Billing, and Quality Management System modules. QBench is a cloud-based Laboratory...

See Software
HSI Donesafe

HSI Donesafe is a no-code, cloud-based EHS software that simplifies safety, compliance, and risk management, transforming complex processes into user-friendly workflows. Trusted by industries worldwide, Donesafe allows organizations to track, manage, and report on essential EHS functions from...

See Software
RegDesk

With the ever-increasing complexity of global medical device registration regulations, more companies are turning to regulatory information management systems software (RIMS software) to streamline the application life cycle and manage medical device applications. RegDesk is the most...

See Software
SAP S/4HANA Cloud Public Edition

SAP Cloud ERP is the premier ERP solution for growth-focused organizations. Seamlessly integrating AI, and predictive analytics, it empowers businesses to digitally transform and streamline processes end to end. Leveraging built-in industry best practices, SAP Cloud ERP accelerates...

See Software