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-What is BRIC-seq?
BRIC-seq, short for 5'-bromo-uridine (BrU) immunoprecipitation chase-deep sequencing analysis, is a novel method in Next Generation Sequencing (NGS) to determine the RNA stability of each transcript. It employs metabolic labeling by BrU of newly synthesized RNAs to chase chronological decreases of BrU-labeled RNA (Pulse-chase labeling), followed by immunoprecipitation with antibody for BrU, construction of cRNA library for High-throughput sequencing and computational data analysis.
-What is BriPy?
BriPy package is a BRIC-seq data analysis tool written in Python. It can calculate RNA half-life for each transcript and draw graphs describing the kinetics of RNA decay. In the future, it will provide several useful modules(e.g. calculating RPKM of each transcript from BAM file or ELAND output file).
-Prerequisites
To use BriPy package, you will need Python and the following extension packages.
Python2.7 or higher
NumPy
SciPy
Matplotlib
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