AutoPrime allows to rapidly design primers for real-time PCR measurement of eukaryotic expression.
By connecting to the EnsEMBL database gene structure information is used to find primers spanning exon borders. This way only expressed sequences are captured. Additionally mispriming on repetitive regions can be avoided using information from RepBase.
Features
- Automated primer design
- Quality control through detailed settings
- Using well-known sources: Primer3, EnsEMBL, RepBase
- Creating your primers since 2003
- Website running as a service
- Perl code available for local execution
Categories
Bio-InformaticsLicense
GNU General Public License version 2.0 (GPLv2)Follow Autoprime
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