We implement a very simple approach. Each read is searched for the junction adapter (CTGTCTCTTATACACATCT) and its reverse complement (AGATGTGTATAAGAGACAG) based on Hamming distance, the entire junction being CTGTCTCTTATACACATCT+AGATGTGTATAAGAGACAG. If a match to either side of the junction is found then the entire 38bp juncton is clipped. This means the algorithm can tolerate an indel error in one of the sides of the junction, but not both. Optionally Smith-Waterman alignment can be turned on with -w which resolves this issue, although the code is less tested.

eg. this will be caught:

CTGTCTCT-TA-TACACATCTAGATGTGTATAAGAGACAG
but not this:

CTGTCTCT-TA-TACACATCTAGATGT-GTATAAGAGACAG

Project Activity

See All Activity >

Follow adapter trimmer

adapter trimmer Web Site

Other Useful Business Software
Forever Free Full-Stack Observability | Grafana Cloud Icon
Forever Free Full-Stack Observability | Grafana Cloud

Our generous forever free tier includes the full platform, including the AI Assistant, for 3 users with 10k metrics, 50GB logs, and 50GB traces.

Built on open standards like Prometheus and OpenTelemetry, Grafana Cloud includes Kubernetes Monitoring, Application Observability, Incident Response, plus the AI-powered Grafana Assistant. Get started with our generous free tier today.
Create free account
Rate This Project
Login To Rate This Project

User Reviews

Be the first to post a review of adapter trimmer!

Additional Project Details

Registered

2017-04-16